Photometric Enzyme Method for Detecting Acrosin Activity in Spermatozoa


  • 4-component kit
  • 1-step 90-minute incubation
  • colorimetric result
  • protease control included
  • 96 determinations per kit
  • 18-month shelf life
  • Background

    Acrosin is a sperm acrosomal protease that has an essential role in the fertilization process.
    Acrosin is released during the acrosome reaction.  It has been shown to be involved in the
    subsequent binding of spermatozoa to the egg's zona pellucida and/or the penetration of
    spermatozoa through the zona pellucida. Low levels of acrosin appear to be associated with
    subfertility and infertility. Studies have further shown that total acrosin activity positively
    correlates with in vitro fertilization rates and a low acrosin activity in an otherwise normal
    ejaculate is associated with an impaired hamster egg penetration.

    The acrosin activity of human ejaculates varies independently from the standard semen parameters
    such as sperm concentration, percent motility, sperm motion characteristics and morphology.
    Thus the enzyme may be an additional marker for human semen quality.

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