ImmunoSpheres® Anti-IgA
          ImmunoSpheres® Anti-IgG
          ImmunoSpheres® Anti-IgM

Sperm bound with Anti-IgG ImmunoSpheres, 
400X. Courtesy of Grace M. Centola.

Bead Method for the Detection of Sperm-Reactive IgA, IgG, IgM and Ig (Heavy
and Light Chains) Antibodies

  • 100 determinations per reagent
  • recommended as a replacement to the ImmunoBeadsTM Test(IBT)
  • improvement over IBT-- see comparison below
  • 18-month shelf life
  • Background Information

    There is considerable evidence that antibodies directed against sperm surface antigens
    exert a fertility-reducing effect.  When antisperm antibodies are present, they bind to the
    surface of the sperm.  Such sperm-bound antibodies may impair sperm movement,
    penetration of the cumulus and/or zona pellucida, the acrosome reaction, interaction of
    sperm and egg as well as identify the sperm for destruction by the immune system.  It
    has also been suggested that sperm antibodies may exert an embryotoxic effect.
    Finally, there is evidence that antibody concentration is correlated directly with the severity
    of sperm functional impairment and inversely with potential fertilizability.

    For these reasons, many reproductive specialists screen sperm and/or serum for anti-sperm antibodies
    before choosing an
    appropriate Assisted Reproductive Technology.

    Comparison of ImmunoSpheres® and ImmunoBeadsTM (IBT)

    ImmunoSpheres and ImmunoBeads mixed on
    a slide, 400X. Courtesy of Grace M. Centola.

    Product Features 
    Beads monodispersed  tend to clump
    Bead Size uniform 3.0um latex beads non-uniform size beads; can be mistaken for cellular debris
    Bead Color  blue for Anti-IgG 
    blue for Anti-IgA
    blue for Anti-IgM
    white for Anti-Ig(H&L)
    Bead Preparation wash each time weigh out and wash each time
    Microscopy bright field or phase contrast phase contrast
    Shelf Life 18 months 1 month once reconstituted
    Package Insert ImmunoSpheres Anti-IgA, Anti-IgG and Anti-IgM Beads

    Package Insert ImmunoSpheres Anti-Ig(H&L) Beads

    MSDS ImmunoSpheres Anti-Ig(H&L) Beads